1. Kaupapa: Ko te whāinga o tēnei tikanga he whakarato i tētahi tikanga paerewa mō ngā mahi ārai mate me te tiaki i ngā rūma parakore.
2. Te whānuitanga o te tono: taiwhanga whakamātautau koiora
3. Tangata Whakahaere: Kaiwhakamātautau Kaiwhakahaere QC
4.Whakamāramatanga: Kāore
5. Ngā mahi ārai haumaru
Me mahi kia tino ātete ki ngā mahi poke hei ārai i te poke o ngā huakita; me whakaweto e ngā kaiwhakahaere te rama UV i mua i te tomo ki te rūma pokekore.
6. Ngā Tikanga
6.1. Me whakarite he rūma pokanga pokekore me he rūma ārai i roto i te rūma parakore. Me eke te ma o te rūma pokanga pokekore ki te akomanga 10000. Me pupuri te pāmahana o roto ki te 20-24°C, ā, me pupuri te haumākū ki te 45-60%. Me eke te ma o te paepae pokekore ki te akomanga 100.
6.2. Me noho ma te rūma parakore, ā, kāore rawa e whakaaetia kia puranga paru hei ārai i te poke.
6.3. Ārai rawatia te poke o ngā taputapu whakamate me ngā rauemi whakatipu katoa. Me mutu te whakamahi i ngā mea kua poke.
6.4. Me whakauru ngā patu huakita kukū mahi ki te rūma parakore, pērā i te otinga cresol 5%, te waipiro 70%, te otinga chlormethionine 0.1%, me ētahi atu.
6.5. Me whakahoromata te rūma parakore, ā, me horoi ki te paturopi tika kia tutuki ai ngā whakaritenga mō te ma o te rūma parakore.
6.6. Me takai pūmau ngā taputapu, ngā rīhi me ngā taonga katoa e tika ana kia kawea ki roto i te rūma parakore, ā, me whakahoromata hoki mā ngā tikanga tika.
6.7. I mua i te tomokanga ki te rūma parakore, me horoi ngā ringa o ngā kaimahi ki te hopi, ki te patu huakita rānei, kātahi ka huri ki ngā kākahu mahi motuhake, ki ngā hu, ki ngā pōtae, ki ngā kopare kanohi me ngā karapu i roto i te rūma ārai (me horoi anō rānei ō rātou ringa ki te 70% etanoru) i mua i te tomokanga ki te rūma parakore. Me mahi ngā mahi i roto i te rūma huakita.
6.8. I mua i te whakamahinga o te rūma parakore, me whakakā te rama ultraviolet i roto i te rūma parakore mō te whakamahana me te whakahoromata mō te neke atu i te 30 meneti, ā, me whakakā te paepae ma mō te pupuhi hau i te wā kotahi. Kia oti te mahi, me horoi te rūma parakore i te wā tika, kātahi ka whakahoromata ki te rama ultraviolet mō te 20 meneti.
6.9. I mua i te tirotiro, me pupuri tonu te takai o waho o te tauira whakamātautau kia kore ai e poke. I mua i te tirotiro, whakamahia he pōro miro waipiro 70% hei patu huakita i te mata o waho.
6.10. I ia mahi, me whakahaere he mana whakahaere kino hei tirotiro i te pono o te mahi ārai-haumanu.
6.11. Ina mimiti wai huakita, me whakamahi he pōro momi hei mimiti. Kaua e pā tika atu ki te kakau witi ki tō waha.
6.12. Me whakahoromata te ngira werohanga ki te mura i mua atu, ā, i muri hoki i ia whakamahinga. Kia whakamatao, ka taea te werohanga i te ahurea.
6.13. Me rumaki ngā ngongo, ngā ipu whakamātautau, ngā rihi petri me ētahi atu taputapu kei roto he wai huakita ki roto i tētahi peere whakahoromata kei roto he 5% te otinga Lysol hei patu huakita, ā, me tango atu, ka horoia i muri i ngā haora 24.
6.14. Mena he wai huakita kua maringi ki runga i te tēpu, i te papa rānei, me riringi tonu te 5% waikawa carbolic, te 3% Lysol rānei ki te wāhi kua poke mō te 30 meneti pea i mua i te rongoā. Ina poke ngā kākahu mahi me ngā pōtae i te wai huakita, me tango tonu atu, ā, me horoi i muri i te whakahoromata i te mamaoa pēhanga teitei.
6.15. Me patu huakita ngā taonga katoa kei roto ngā huakita ora i mua i te horoinga i raro i te wai. Kāore e whakaaetia kia poke te waipara.
6.16. Me tirotiro te maha o ngā koroni i roto i te rūma parakore i ia marama. Me te tūwhera o te paepae ma, tangohia he maha o ngā rihi petri parakore me te diameter o roto o te 90 mm, ka werohia kia 15 ml pea o te reo ahurea agar matūkai kua rewahia, kua whakamataohia ki te 45°C pea. Whai muri i te whakapakeketanga, whakatakotoria ki raro i te 30 ki te 35. Whakatōngia mō ngā hāora 48 i roto i te incubator ℃. Whai muri i te whakamātautau i te parakore, tangohia kia 3 ki te 5 ngā pereti ka whakatakotoria ki te taha maui, waenganui me te taha matau o te tūranga mahi. Whai muri i te whakatuwheratanga o te taupoki me te whakaatu i a rātou mō ngā meneti 30, whakatakotoria ki raro i roto i te incubator 30 ki te 35°C mō ngā hāora 48 ka tangohia. tirohia. Ko te maha toharite o ngā huakita rerekē i runga i te pereti i roto i te wāhi ma o te akomanga 100 kaua e neke atu i te 1 koroni, ā, ko te maha toharite i roto i te rūma ma o te akomanga 10000 kaua e neke atu i te 3 koroni. Mena ka hipa te rohe, me tino horoia te rūma parakore kia tutuki rā anō ngā whakaritenga.
7. Tirohia te upoko (Tikanga Tirotiro Hauora) i roto i te "Ngā Tikanga Tirotiro Hauora Rongoā" me te "Ngā Tikanga Whakahaere Paerewa Haina mō te Tirotiro Rongoā".
8. Tari Tohatoha: Tari Whakahaere Kounga
He aratohu hangarau mō te rūma ma:
I muri i te whiwhinga i tētahi taiao parakore me ngā rauemi parakore, me pupuri tonu tātou i tētahi āhua parakore kia taea ai te ako i tētahi moroiti motuhake e mōhiotia ana, te whakamahi rānei i ā rātou mahi. Ki te kore, ka taea e ngā momo moroiti mai i waho te whakauru ngāwari. Ko te āhuatanga o te whakaranu i ngā moroiti koretake mai i waho e kiia ana ko ngā huakita poke i roto i te moroiti koiora. Ko te aukati i te poke he tikanga nui i roto i te mahi moroiti koiora. Ko te whakahoromata katoa i tētahi taha me te aukati i te poke i tētahi atu taha e rua ngā āhuatanga o te tikanga aseptic. Hei tāpiri, me ārai tātou i ngā moroiti e akohia ana, inā koa ko ngā moroiti mate, ko ngā moroiti i hangaia ira rānei kāore i te noho i roto i te taiao, kei mawhiti mai i ā tātou ipu whakamātautau ki te taiao o waho. Mō ēnei kaupapa, i roto i te moroiti koiora, he maha ngā tikanga.
Ko te rūma parakore he rūma iti i hangaia mō te taiwhanga moroiti. Ka taea te hanga ki ngā rau me te karāhe. Kaua te wāhi e nui rawa, kia 4-5 mita pūrua te whānui, ā, kia 2.5 mita te teitei. Me whakatū he rūma ārai i waho o te rūma parakore. Kaua te tatau o te rūma ārai me te tatau o te rūma parakore e anga atu ki te taha kotahi hei ārai i te rere o te hau mai i ngā huakita rerekē. Me ārai te hau o te rūma parakore me te rūma ārai. Me whai taputapu tātari hau ngā taputapu hau o roto. Me maeneene te papa me ngā pakitara o te rūma parakore, me uaua ki te pupuri paru, ā, me ngāwari ki te horoi. Me papatahi te mata mahi. Kei te rūma parakore me te rūma ārai ngā rama ultraviolet. Kotahi mita te tawhiti o ngā rama ultraviolet i roto i te rūma parakore mai i te mata mahi. Me mau ngā kaimahi e tomo ana ki te rūma parakore i ngā kākahu me ngā pōtae kua parakore.
I tēnei wā, ko ngā rūma parakore kei roto i ngā wheketere moroiti, engari ko ngā taiwhanga whānui e whakamahi ana i te paepae ma. Ko te mahi matua o te paepae ma ko te whakamahi i te taputapu rere hau laminar hei tango i ngā puehu ririki tae atu ki ngā moroiti i runga i te mata mahi. Mā te taputapu hiko ka taea e te hau te haere mā te tātari hepa, kātahi ka tomo ki te mata mahi, kia mau tonu ai te mata mahi i raro i te mana o te hau parakore e rere ana. Heoi anō, he ārai hau tere-tere kei te taha e tata ana ki waho hei ārai i te hau huakita o waho kei tomo.
I ngā wāhi uaua, ka taea hoki te whakamahi i ngā pouaka parakore rakau hei whakakapi i te paepae ma. He māmā te hanganga o te pouaka parakore, ā, he ngāwari ki te neke. E rua ngā kōhao kei mua o te pouaka, e āraia ana e ngā tatau pana-tō ina kore e whakamahia. Ka taea e koe te totoro i ō ringa ki roto i te wā e whakamahia ana. Kei te taha o runga o mua he karāhe hei whakahaere i te mahi o roto. He rama ultraviolet kei roto i te pouaka, ā, ka taea te whakauru i ngā taputapu me ngā huakita mā roto i te tatau iti i te taha.
I tēnei wā, ehara i te mea he mahi nui noa iho ngā tikanga whakahaere aseptic i roto i ngā rangahau me ngā tono moroiti, engari he whānuitia hoki te whakamahinga i roto i ngā hangarau koiora maha. Hei tauira, ko te hangarau transgenic, ko te hangarau paturopi monoclonal, me ētahi atu.
Wā tuku: Maehe-06-2024